Xu  Jianqin, Zhang  Nuowei, Guo  Shining, Li  Huanrong, Li  Jiefeng, Xu  Xiaolong, Wan  Changrong, Zhao  Hong, Liu  Faqiang, Zan  Junlan, Wang  Bin, 
    Effects of Sijunzi Decoction on Small Intestinal T Lymphocyte Subsets Differentiation in Reserpine Induced Spleen Deficiency Rats,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 21,
    2013,
    Pages 1574-1582,
    ISSN 1680-5593,
    javaa.2013.1574.1582,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1574.1582)
    Abstract: Sijunzi decoction (SJZ), composed of Dangshen (<I>Codonopsis 
  pilosula</I> Nannf.), Baizhu (<I>Atractylodes macrocephala</I> Koidz.), Fuling 
  (Tuckahoe) and Gancao (<I>Radix glycyrrhiza</I>) is well known as a classical 
  Chinese traditional and herbal medicamentum aiming directly at spleen deficiency 
  in Traditional Chinese Medicine (TCM). The present research is to evaluate the 
  regulatory effect of SJZ on small intestinal T lymphocyte subsets differentiation 
  in reserpine-induced spleen deficiency rats. Reserpine induced spleen deficiency 
  rats were orally administrated with SJZ (0.2, 0.6 and 1.0 mL/200 g, body weight) 
  once a day for a period of 5 days. Clinical symptoms including body weight were 
  observed every day, the small intestine damage and recovery by SJZ were detected 
  by electron microscope scanning, small intestine lymphocytes were separated 
  for T lymphocyte subsets detection by flow cytometry and T lymphocyte related 
  cytokine expression was studied using quantitative real time PCR. Results showed 
  that proper dose of SJZ can significantly promote the recovery of body weight 
  and small intestine damage as well as regulate the differentiation of CD3<SUP>+</SUP>, 
  CD3<SUP>+</SUP>CD4<SUP>+</SUP> T lymphocyte subsets and the expression of type-1 
  cytokines (IL-2, IFN-&#947; and IL10). Besides, a significant CD4&#175; CD8&#175; 
  T lymphocyte subset was found in reserpine treated and high dose SJZ administrated 
  rats. Data from this study proved that SJZ possess a significant promoting effect 
  on the amelioration of spleen deficiency, CD4<SUP>+</SUP> T lymphocyte and cytokines 
  related with regulation of T lymphocyte differentiation would be key targets 
  of SJZ on spleen deficiency.
    Keywords: Sijunzi decoction;T lymphocyte subsets;reserpine induced;spleen deficiency;small intestine