Jianglong  Yuan, Yong  Jin, Bing  Zhu, Wei  Wang, Yu  Ren, Haiqing  Wu, Fei  Hao, Mingtu  Nuo, Muzi  Jin, Zhigang  Wang, Ming  Chang, Dongjun  Liu, Xudong  Guo, 
    Somatic Cell Nuclear Transfer Efficiency Associated with Donor Cell Types in the Cashmere Goat,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 24,
    2012,
    Pages 4578-4584,
    ISSN 1680-5593,
    javaa.2012.4578.4584,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4578.4584)
    Abstract: The efficiency of donor cell types in cashmere goat Somatic 
  Cell Nuclear Transfer (SCNT) is undefined. In this study, SCNT was performed 
  using Caprine fetal Fibroblast Cells (CFCs), Caprine Ear Fibroblast Cells (CEFCs) 
  and Transgenic Caprine Fetal Fibroblast Cells (TCFCs) as donor cells to compare 
  the influences of cell type, transgene and sex of nuclear donor fibroblast cells 
  on the SCNT efficiency including fusion and cleavage rates of reconstructed 
  embryos and the birth, postnatal survival and mortality rates of cloned kids. 
  A total of 4,943 reconstructed embryos were obtained. Among them, 3,949 embryos 
  were fused and 3,737 embryos were cultured <I>in vitro</I> leading to a total 
  of 3,094 cleavage embryos. Furthermore, embryo transplantation analysis was 
  conducted on 1,873 cloned embryos with relatively normal morphology. A total 
  of 368 recipient goats were transplanted and 48 goats were born of which 35 
  goats survived.
    Keywords: Cashmere goat;somatic cell nuclear transfer efficiency;cell type;embryo;China