Li-Guo  Yang, Ai-Xin  Liang, Fang-Xiao  Dong, Xuan  Luo, Xue  Yu, Li  Han, Yan-Guo  Han, Ai-Zhen  Guo, 
    Immunization Procedures of Δcrp Δasd Double Deleted Attenuated 
  Salmonella Enterica Serovar Typhimurium delivering Somatostatin Antigen in Mice,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 24,
    2012,
    Pages 4526-4532,
    ISSN 1680-5593,
    javaa.2012.4526.4532,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4526.4532)
    Abstract: In order to estimate the optimal immunization procedures for 
  a novel somatostatin vaccine carried by Δcrp Δasd double deleted attenuated 
  Salmonella enterica serovar Typhimurium. Mice were vaccinated with somatostatin 
  vaccine strain (C500/pVGS/2SS-asd) by using three immunization dosages and three 
  alternative schedules. The specific antibody response was evaluated by indirect 
  ELISA Method. The results showed that 0.2x10<SUP>10</SUP> CFU per mouse was 
  the optimal immunization dosage to induce specific IgG response. Interestingly, 
  the booster groups either 2 weeks interval or 4 weeks interval after the first 
  immunization were not improving the level of specific IgG response as expected 
  compared with only injection one dose groups. Furthermore, the different interval 
  period of booster injections also affected the immune response, antibody levels 
  of booster given 4 weeks interval was much higher than that booster given 2 
  weeks interval. Similar results were obtained on body weight gains analysis. 
  The results of body weight gains of immunized mice also showed that the most 
  effective immunization dosage is 0.2x10<SUP>10</SUP> CFU per mouse and the optimal 
  immunization number is a single dose. These observations have a very important 
  application in the field of animal production.
    Keywords: Somatostatin;Salmonella;dose;interval;mice