X.M.  Song, S.S.  Lu, M.  Wang, Q.Y.  Li, D.  Li, X.G.  Yang, Y.Q.  Lu, M.  Zhang, K.H.  Lu, 
    Addition of Glutathione to Semen Extender During Sperm Sorting can Improve <i>in vitro</i> Embryonic Development and <i>in vivo</i> Fertility 
  in Buffalo,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 23,
    2012,
    Pages 4482-4488,
    ISSN 1680-5593,
    javaa.2012.4482.4488,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4482.4488)
    Abstract: This study evaluated the effects of Glutathione (GSH) supplemented 
  in the semen extender during the buffalo sperm sorting process on sperm quality, 
  embryonic development after IVF and pregnancy rate after AI. The percentage 
  of sperm motility, plasma membrane integrity and DNA fragmentation were detected 
  by flow cytometry or by microscopy in stained, sorted and frozen semen treated 
  with or without 0.75 mM GSH during the flow sorting procedure. The cleavage 
  and blastocyst rates were examined at day 2 and 6-8 after IVF with frozen semen 
  treated with or without 0.75 mM GSH. Pregnancy diagnosis was determined by transrectal 
  palpation at 90 day after AI with frozen semen treated with or without 0.75 
  mM GSH. The percentage of sperm with Progressive Motility (MP, %) was significantly 
  higher (p&lt;0.05) in sorted semen supplemented with 0.75 mM GSH than that in 
  the control. The percentages of moribund, dead and Phosphatidylserine (PS) translocated 
  sperm detected by flow cytometry were significantly decreased (p&lt;0.05) in 
  frozen semen supplemented with GSH compared to the control. Higher blastocyst 
  and pregnancy rates (p&lt;0.05) were found after IVF and AI with frozen sperm 
  treated with 0.75 mM GSH than that in the control group. In conclusion, addition 
  of 0.75 mM GSH to the semen extenders (stained, sorted and frozen) during the 
  sperm sorting process can improve sperm quality <I>in vitro</I> embryonic development 
  and <I>in vivo</I> fertility after AI thus indicating potential for commercial 
  application in buffalo sperm sorting.
    Keywords: Reduced glutathione;sperm quality;embryonic development;pregnancy rate;sorted semen;buffalo