Haijin  Liu, Yi  Liu, Bingqian  Wang, Zhaoting  Fan, 
    Expression of Proliferation Cell Nuclear Antigen (PCNA) in Peripheral Erythrocytes of Triploid Rainbow Trout <i>Oncorhynchus mykiss </i>(Walbaum),
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 23,
    2012,
    Pages 4451-4454,
    ISSN 1680-5593,
    javaa.2012.4451.4454,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4451.4454)
    Abstract: The proliferation of erythroid cells in peripheral blood was 
  detected by anti-PCNA monoclonal antibody (PC10) with a means of immunocytochemistry 
  in diploid and triploid rainbow trout. The rate of PCNA positive erythroid cells 
  in triploidy peripheral blood was detected by flow cytometry. The erythrocytes 
  which were positive for PCNA were confined in the cells with high nuclear-cytoplasmic 
  ratio. About 6.4% erythrocytes were found positive for PCNA in triploid rainbow 
  trout while only 0.4% in diploids. And the PCNA positive rate of the erythrocytes 
  was 0.37% in diploids and 8.39% in triploids in average. PCNA, a proliferating 
  marker is found to be expressed only in immature cells in the peripheral erythrocytes 
  of triploid rainbow trout. This suggests that peripheral erythrocytes with the 
  ability of proliferating are immature cells.
    Keywords: Rainbow trout;triploid;PCNA;immature erythrocyte;erythroid cells;China