Lifang  Si, Yun  Xue, Xiangchao  Cheng, Chunsheng  Gao, Zhanqin  Zhao, Shulin  Chen, Jin  Zhao, 
    The Effects of Interferon-Gamma (IFN-γ) on the Expression of IL-10 
  in Hypothalamic Pituitary Ovarian Axis and Peripheral Blood,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 23,
    2012,
    Pages 4310-4314,
    ISSN 1680-5593,
    javaa.2012.4310.4314,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4310.4314)
    Abstract: The aim of this research was to confirm the effect of IFN-γ 
  on the expression level of IL-10 in hypothalamic pituitary ovarian axis and 
  peripheral blood of rats during early pregnancy. The rats (gestational day 9) 
  were randomly divided into 3 groups: test group 1 (vaginal muscular injection 
  with 2.5x10<SUP>4</SUP> U IFN-&#947; per rat), test group 2 (vaginal muscular 
  injection with 7.5x10<SUP>4</SUP> U IFN-&#947; per rat) and control group (vaginal 
  muscular injection with normal saline). About 48 h after injection, the hypothalamus, 
  pituitary, ovary, uterus and peripheral blood were collected to research the 
  effect of exogenous IFN-&#947; on the expression level of IL-10 by morphological 
  observation, immunohistochemical SP and ELISA. The results showed that injection 
  of IFN-&#947; (in the dose of both 2.5x10<SUP>4</SUP> U and 7.5x10<SUP>4</SUP> 
  U per rat) decreased the expression of IL-10 in hypothalamic periventricular 
  nucleus, hypothalamic supraoptic nucleus, nucleus pre-opticus magno cellularis, 
  medial preoptic nucleus, lateral preoptic nucleus, suprachiasmatic nucleus, 
  nucleus arcuatus hypothalamic, adenohypophysis, corpus luteum, stromal cells 
  in uterine decidua, utricular glands and peripheral blood. These findings suggest 
  that IFN-&#947; could down regulate the expression of IL-10 in hypothalamic-pituitary-ovarian 
  axis and peripheral blood at a does independent manner.
    Keywords: IL-10;IFN-y;immunohistochemical;hypothalamic pituitary ovarian axis;pregnant rats