Ho-Seong  Cho, Nag-Jin  Choi, Byoung-Yong  Park, Chul  Park, Young-Jun  Kim, 
    A Rapid Colorimetric Loop-Mediated Isothermal Amplification Assay for the Detection of <I>Lawsonia intracellularis </I>in Pigs,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 22,
    2012,
    Pages 4258-4262,
    ISSN 1680-5593,
    javaa.2012.4258.4262,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.4258.4262)
    Abstract: A colorimetric Loop-mediated isothermal Amplification (LAMP) 
  assay with hydroxy naphthol blue was designed for the detection of <I>Lawsonia</I> 
  (L.)<I> intracellularis</I> which is an important pathogenic bacteria that causes 
  animal diseases. A set of six primers was designed to target the aspartate ammonia-lyase 
  (<I>asp</I>A) gene. Serial 10 fold dilutions of cultured <I>L. intracellularis</I> 
  and spiked feces were used for LAMP optimization. The lower limit of the linear 
  range of the assay in <I>L. intracellularis</I> was 10<SUP>1</SUP> <I>L. intracellularis</I> 
  which is ten times more sensitive than conventional PCR. Based on testing in 
  210 porcine fecal samples using LAMP and conventional PCR, the agreement quotients 
  between LAMP and conventional PCR was 0.92. These results suggest that colorimetric 
  LAMP is a simple method for <I>L</I>. <I>intracellularis </I>detection in porcine 
  fecal samples.
    Keywords: Lawsonia intracellularis;loop-mediated isothermal amplification;LAMP;porcine proliferative enteropathy;pigs