ZhiZhong  Jing, XuePeng  Cai, XiaoBing  He, YongXiang  Fang, Shuang  Zeng, YaDong  Zhen, HuaiJie  Jia, GuoHua  Chen, 
    Characterization of Interferon-α1 of Yak,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 21,
    2012,
    Pages 3978-3983,
    ISSN 1680-5593,
    javaa.2012.3978.3983,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3978.3983)
    Abstract: The yak (<I>Bos grunniens</I>) is an important grazing livestock in Western of China. In this study, the full length of <I>yIFN-a1</I> gene was cloned then the recombinant yIFN-a1 was expressed in yeast expression system. <I>In vitro</I> bioactivity of IFN-&#945;1 was determined by inhabition infection BHK cell against Vesicular Stomatitis Virus (VSV). The results showed that cDNA contains an Open Reading Frame (ORF) of 570 base pairs encoding a protein of 189 amino acid (aa) precursor with a signal peptide of 23 aa. The homology of the yIFN-a1 was up to 65.3~94.4% with other species at the amino acid levels. The recombinant yIFN-a1 was recognized in Western blot by mAb against human IFN-&#945;1. The activity unit of the purified recombinant yIFN- a1 protein was 1.16&times;10<SUP>5</SUP> IU mg<SUP>-1</SUP> by against Vesicular Stomatitis Virus (VSV) antiviral assay <I>in vitro</I>. This made a basis for the development of anti-virus medicine of yak in the future.
    Keywords: cloning;interferon-a1;Yak (Bos grunniens);antiviral activity assay;Pichia pastoris