Shiwei  Ma, Wenge  Hu, Chuangfu  Chen, Yuanzhi  Wang, Yan  Ren, Xudong  Cao, Jinliang  Sheng, 
    Characterization of &#946;-Actin Promoter of <I>Leuciscus merzbacheri</I>,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 20,
    2012,
    Pages 3849-3854,
    ISSN 1680-5593,
    javaa.2012.3849.3854,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3849.3854)
    Abstract: <I>Leuciscus merzbacheri</I> is a unique vulnerable indigenous 
  fish which is only distributed in the Junggar Basin, Xinjiang. In this study, 
  researchers cloned two <I>L. merzbacheri</I> &#946;-actin promoter fragments 
  of different length: SZ11 and SZ21 and analyzed their structural features. The 
  mammalian expression vector pEGFP-N1 was used to construct eukaryotic expression 
  vectors &#946;1 pEGFP-N1-AFP III and &#946;2 pEGFP-N1-AFP III in which fish 
  type III antifreeze protein gene was used as the structural gene. The results 
  showed that the cloned two fragments of &#946;-actin promoters had the ability 
  to drive the expression of the green fluorescent protein gene in BHK-21 cells. 
  These data suggest that the vectors researchers constructed based on &#946;-actin 
  promoter could be exploited as all fish recombinant eukaryotic expression vector.
    Keywords: Leuciscus merzbacheri;promoter analysis;B-actin gene;green fluorescent protein;China