Kai-Yu  Wang, Hai  Lian, De-Fang  Chen, Jun  Wang, Ling-Yuan  Huang, Cheng-Wei  Li, 
    Prokaryotic Expression of <I>p1</I> Gene of <I>Yersinia ruckeri</I> Isolated 
  from Channel Catfish (<I>Ictalunes punctatus</I>) and Optimization of Expression Conditions,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 20,
    2012,
    Pages 3800-3805,
    ISSN 1680-5593,
    javaa.2012.3800.3805,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3800.3805)
    Abstract: The <I>p1</I> gene of <I>Yersinia ruckeri</I> (<I>yrp1</I>) 
  which was isolated from channel catfish was amplified by PCR with specific primers 
  and inserted into pMD19-T vector. The positive recombinant plasmid was selected 
  and sequenced. Then, the <I>yrp1</I> gene was subcloned into pET-32a (+) vector 
  and transformed into BL21 (DE3) followed by induction with IPTG and detection 
  with SDS-PAGE. Optimization of the induction conditions were conducted. The 
  results showed that the recombinant protein with a molecular mass of about 72 
  kDa was mostly packaged into inclusion bodies. The optimization of induction 
  process conditions led us to perform the fusion protein induction at 37&deg;C 
  for 4 h with 0.8 mM IPTG.
    Keywords: Yersinia ruckeri;p1 gene;prokaryotic expression;optimization;China