Jin-Ru  Cao, Hong-Biao  Weng, Cheng-Liang  Gong, Ai-Hong  Ye, Wen-Lin  Zhou, Li-Hua  He, Ren-Yu  Xue, Guang-Li  Cao, Yong-Qiang  Wang, 
    Expression of the B Subunit of <I>Escherichia coli</I> Heat-Labile Enterotoxin in Transformed <I>Bombyx mori</I> BmN Cells,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 20,
    2012,
    Pages 3785-3791,
    ISSN 1680-5593,
    javaa.2012.3785.3791,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3785.3791)
    Abstract: The non-toxic B subunit of<I> Escherichia coli</I> heat-labile 
  enterotoxin (LTB) is a potent mucosal immunogen and immunoadjuvant for coadministered 
  antigens. To obtain transformed silkworm cell line stably expressing LTB, researchers 
  fused the LTB coding sequence, neomycin-resistance gene (<I>Neo<SUP>R</SUP></I>) 
  and <I>gfp</I> gene into piggyBac-based transponson vector and transduced into 
  silkworm BmN cells. After screening against antibiotic G418, the positive rate 
  of cells emitting green fluorescence was 70.79%. PCR detection indicates the 
  existence of exogenous LTB coding sequence, Neo<SUP>R</SUP> and gfp in the transformed 
  cell genome. Western blot analysis also confirms the predicated ~60 kDa band 
  of LTB protein. These results demonstrated that the strategy was practicable.
    Keywords: Escherichia coli heat-labile enterotoxin;piggyBac transposon;Bombyx mori;BmN cell;genetic transformation