Wenjin  Zhu, Yongmei  Su, Yanfang  Liu, Xuemin  Guan, Jing  Ni, Jianhua  Wu, 
    Allelic Polymorphism Identified and Analysis on the Fifth Exon of Chinese Indigenous Donkey <I>GH</I> Gene by PCR-SSCP,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 20,
    2012,
    Pages 3713-3716,
    ISSN 1680-5593,
    javaa.2012.3713.3716,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3713.3716)
    Abstract: Growth of animal is largely regulated by Growth Hormone (GH). 
  In this study the objective was to investigate variations in the fifth exon 
  of <I>GH</I> gene and their polymorphism in 5 Chinese indigenous donkeys by 
  PCR-SSCP and DNA Sequencing Methods. The results showed that there was a transversion 
  at nucleotide position 1802 of <I>GH</I> gene in the 5th exon named as GH-exon5-G1802C 
  which led to a conservative Lysine to Asparagine substitution at amino acid 
  position 205. In detail, two different alleles, A and B were identified and 
  three genotypes were observed, AA, AB and BB with the frequency distribution 
  of allele B from 0.5962-0.7344 in analyzed populations. The genetic diversity 
  analysis revealed that all PIC values were between 0.3140 and 0.3656, implying 
  that this locus within <I>GH</I> gene possessed moderate genetic diversity in 
  5 Chinese indigenous donkeys. The &#967;<SUP>2</SUP>-test showed that GZ donkey 
  was significant deviation, DZ donkey was no significant deviation from Hardy-Weinberg 
  equilibrium, GL, JN and XJ donkey was not in agreement Hardy-Weinberg equilibrium. 
  The results confirmed that there were polymorphisms in the exon 5 of <I>GH</I> 
  gene.
    Keywords: Donkey;GH gene;exon3;polymorphism;PCR-SSCP