Cheng  Peng, Min  Dai, Feng  Wan, Fu  Peng, 
    Antibacterial Activity and Mechanism of <I>Pogostemon cablin</I> Against Bacteria from Milk of Dairy Cows Suffering with Mastitis,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 18,
    2012,
    Pages 3289-3297,
    ISSN 1680-5593,
    javaa.2012.3289.3297,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3289.3297)
    Abstract: Minimum Inhibitory Concentrations (MICs) of 3 extracts and oil from <I>Pogostemon cablin </I>against isolates from milk produced by dairy cow with mastitis were determined by Agar Dilution Method. Water extract had low activity; ethanol extract and ethanol-water extract had moderate effects having MICs ranging from 25.0-100.0, 12.5-100.0 mg mL<SUP>-1</SUP>, respectively. Oil had strong activity against Gram-positive and Gram-negative isolates with MICs ranging from 0.2-8.2 mg mL<SUP>-1</SUP>. Oil also, showed strong efficacy in mouse peritonitis model infected with <I>S. aureus</I> or <I>E. coli</I>. Their actions were dose-dependent. Obvious morphological changes were observed by TEM in oil-treated <I>E. coli</I> or <I>S. aureus</I> such as a remarkable electron-light region small electron-dense granules disturbed cytoplasmic membrane and abnormal cell division. In conclusion, antibacterial substance of <I>P. cablin</I> was oil which had broad-spectrum antibacterial activities so to make it a potential candidate as antibacterial agent to treat bovine mastitis.
    Keywords: Pogostemon cablin;essential oil;bovine mastitis;in vitro and in vivo antibacterial activity;antibacterial mechanism;China