Yingji  Wu, Boyang  Yu, Fenhua  Luo, Taodi  Liu, Daiyan  Liu, Linhong  Liu, Yan  Zhang, 
    Preparing Rat Spermatogonial Transplantation Recipient by Treating the Pregnant Female Rats with Busulfan,
    Journal of Animal and Veterinary Advances,
    Volume 11,Issue 17,
    2012,
    Pages 3036-3044,
    ISSN 1680-5593,
    javaa.2012.3036.3044,
    (https://makhillpublications.co/view-article.php?doi=javaa.2012.3036.3044)
    Abstract: Successful spermatogonial stem cells transplantation is largely dependent on the preparation of the recipient. In the present study, to acquire more efficient rat recipients, the response of pregnant female rats to treatment with 7.5-12.5 mg busulfan per kg of body weight was determined in terms of the birth rate, survival rate, body weight, testicular mass, histology, expression of certain germ cell genes as measured by real time PCR and fertility rate of male offspring. The analysis suggested that a dosage of 10 mg busulfan per kg administered to pregnant female rats at 13.5 day post pregnancy effectively prepares male pups to be recipients because it leads to the maximal deletion of endogenous spermatogonial stem cells with minimal effects on the recipients’ health.
    Keywords: Busulfan;germ cells;infertility;rat;spermatogonial transplantation