Uyunbilig  Borjigin, Xin  Zhou, Xuejie  Han, Rongfeng  Li, Muren  Herrid, Shorgan  Bou, 
    Enrichment and Short Term Culture of the Ovine Gonocyte,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 22,
    2011,
    Pages 2936-2942,
    ISSN 1680-5593,
    javaa.2011.2936.2942,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.2936.2942)
    Abstract: The aim of this study was to investigate the effects of two types of ovine testis cells population as feeder cell on <I>in vitro</I> culture of the enriched ovine gonocytes. The feeder cell populations were prepared from 5-6 months old ovine testis. The 1-2 months old neonatal rams were used to isolate germ cells through a two step enzymatic digestion followed by differential plating for SSCs enrichment. Isolated and enriched cells were characterized by using PLZF and VASA antibody. During the 1st week of culture, gonocyte formed pairs and chains of type A spermatogonia. After 1 week, colonies started to increase in size. About 2 weeks later, more colonies in type II feeder group kept undifferentiated and looks more effective regarding colony formation of spermatogonia compared with type I feeder group in the ovine spermatogonial stem cell culture system.
    Keywords: Ovine;gonocyte;cell culture;immunostaining;spermatogonia;Australia