Xin  Wei, Wei  Jian-Zhong, Huang  Xiao-Hui, Yin  Zong-Jun, Sun  Pei, Li  Yu, 
    The Research of KRG Serotyping and ERIC-PCR and PCR-RFLP Genotyping of <I>Haemophilus parasuis </I>Isolates from Anhui Province of China,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 20,
    2011,
    Pages 2669-2674,
    ISSN 1680-5593,
    javaa.2011.2669.2674,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.2669.2674)
    Abstract: To investigate the characteristics of serology and molecular epidemiology of <I>Haemophilus parasuis </I>in Anhui area, this research adopts KRG serotyping and ERIC-PCR and PCR-RFLP genotyping to conduct systematic analyses on 18 HPS isolates from Anhui. The result shows that 55.6% bacterial strains can be serotyped where the most prevalent serotype is type 4 (27.78%) followed by type 14 (16.67%) and type 13 (11.11%). About 44.4% bacterial strains cannot be serotyped. With ERIC-PCR analyses, 18 HPS isolates contain 4-9 bands with the size ranging from 100-2000 bp and they belong to 11 different genotypes where IV is the dominant genotype (27.78%) followed by type 2 (16.67%) and type 1 (11.11%). With PCR-RFLP analyses, 18 HPS isolates lie in 6 different genotypes where the most prevalent genotype in Anhui area is DBP (44.4%) followed by BAE (22.22%) and BBB (16.67%). The result revels that HPS prevails in pigs in Anhui area and both its serotypes and genotypes are in polymorphism distribution. The result also shows that KRG serotypes, ERIC-PCR genotypes and PCR-RFLP genotypes of HPS are not significantly associated with each other but ERIC-PCR genotype is associated with the sources of bacterial strains with the same serotype. Compared with PCR-RFLP, ERIC-PCR better uncovers the genetic relationships of bacterial strains and hence reveals the genetic distance among different bacterial strains which can be especially applied to the inter-pig and inter-farm epidemic conditions of pathogenium.
    Keywords: PCR-RFLP;ERIC-PCR;serotyping;Haemophilus parasuis;genotyping;China