Dongsheng  He, Zhangyong  Ning, Xiaoli  Yu, Ming  Liao, Kangning  Li, Yanzong  Zhao, 
    Porcine IFN-&#8226; is Biologically Active and Secreted in the Yeast <I>Pichia 
  pastoris</I> Expression System,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 18,
    2011,
    Pages 2450-2455,
    ISSN 1680-5593,
    javaa.2011.2450.2455,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.2450.2455)
    Abstract: Porcine Interferon (PoIFN) is a multifunctional secretory 
  protein produced by animal immune cells. IFN signaling activates host cells 
  resulting in anti-viral and -tumor immune regulation in addition to other biological 
  functions. Here, the coding region of PoIFN-&#8226; was amplified from porcine 
  genomic DNA by Polymerase Chain Reaction (PCR) and was cloned into the yeast 
  <I>Pichia pastoris</I> expression system. This resulted in high levels of gene 
  expression. Furthermore, expression of PoIFN-&#8226; was successfully detected 
  by Western-blot. The relative molecular weight of the product was 25 and 27 
  kDa. The recombinant PoIFN-&#8226; exhibited cross-species antiviral activity 
  in Marc-145 and BHK-21 mammalian cell lines. In summary, the results demonstrate 
  that recombinant PoIFN-&#8226; cloned into <I>P. pastoris</I> is expressed, 
  secreted and biologically active in mammalian cells.
    Keywords: Porcine interferon-• (PoIFN-•);Pichia pastoris;expression;activity;cells;biologically active