Ali  Sorucu, Erdem  Arslan, Murat  Cengiz, 
    <I>In vitro</I> Pharmacodynamics of Enrofloxacin Against an <I>Escherichia coli gyrA</I> Mutant,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 18,
    2011,
    Pages 2384-2388,
    ISSN 1680-5593,
    javaa.2011.2384.2388,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.2384.2388)
    Abstract: The aim of this study was to investigate <I>in vitro</I> the pharmacodynamics of Enrofloxacin (ENR) against an <I>Escherichia coli gyrA</I> mutant (<I>E. coli </I>MT/128). Broth microdilution testing was used to determine the Minimum Inhibitory Concentration (MIC) amd multi-step resistance selection was performed until reaching 1 &#956;g mL&#8226;<SUP>1</SUP> MIC. For time-kill experiments, colony counts were determined by plating each diluted sample onto Plate Count Agar and an integrated pharmacokinetic/pharmacodynamics area measure (log ratio area) was applied to all cfu data. A single C-T mutation was found in gyrA at codon 83. Concentration-dependent bacterial killing was observed for <I>E. coli </I>MT/128. Bactericidal activity for this strain was achieved within 4 h at concentrations &#8226; 8 times the MIC with no significant regrowth by 24 h.
    Keywords: Turkey;Pharmacodynamic of enrofloxacin;Escherichia coli;C-T mutation;microdilution;significant