Yuxin  Cheng, Xunsheng  Pang, Zhihua  Cai, Shenghe  Li, Deyi  Liu, Like  Wang, 
    Polymorphism Identification of<I> IRF6</I> Gene and it Associate with Immune Trait in Three Pig Populations,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 16,
    2011,
    Pages 2095-2098,
    ISSN 1680-5593,
    javaa.2011.2095.2098,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.2095.2098)
    Abstract: In this study, researchers chose the Interferon Regulatory Factor 6 (<I>IRF6</I>) gene as a candidate gene to evaluate its effect on porcine immune traits. Using the PCR-RFLP analysis and sequencing, a SNP (HQ026023:1383 G&gt;C) in exon7 of <I>IRF6</I> gene was detected. In addition, a further analysis of SNP genotypes associated with immune traits including IFN-&#947; and IL10 concentrations in serum was carried out in three pig breeds including Large White, Landraces and Chinese indigenous breed Dingyuan Black pig. Results showed that the SNP of <I>IRF6 </I>gene effects on level of IFN-&#947; and IL10 (day 20) in serum presented highly significances (p = 0.0264; p = 0.0238) and as for the ratio of IFN-&#947; to IL10 (day 20) in serum, the p-value was 0.0341 which also achieved a significant level. All the results could possibly indicate that the <I>IRF6</I> gene should be regarded as a molecular marker gene for genetic selection associated with these immune traits in the pig breeding program.
    Keywords: association analysis;PCR-RFLP;polymorphism;IRF6 gene;Pig;China