Zhi-Fa  Wen, Xiang  Chen, Shi-Zhong  Geng, Qiang  Fang, Zhi-Ming  Pan, Xin-An  Jiao, 
    A Simpler Method for the Efficient and Precise Deletion of Genes in<I> Salmonella </I>sp.,
    Journal of Animal and Veterinary Advances,
    Volume 10,Issue 16,
    2011,
    Pages 2090-2094,
    ISSN 1680-5593,
    javaa.2011.2090.2094,
    (https://makhillpublications.co/view-article.php?doi=javaa.2011.2090.2094)
    Abstract: A simpler method for the efficient and precise deletion of genes in <I>Salmonella </I>sp. was developed. To demonstrate this approach, the <I>prgH </I>gene of <I>Salmonella typhimurium </I>SL7207 was deleted by homologous recombination with a temperature-sensitive plasmid containing a cassette that two DNA fragments as homologous arms flanking chloramphenicol resistance gene (<I>Cm<SUP>R</SUP></I>) which replaced the <I>prgH </I>gene. During screening mutant at 44&deg;C, the temperature-sensitive plasmid was lost easily only the mutant which<I> prgH</I> gene was replaced by <I>Cm<SUP>R</SUP></I> gene could grow in the LB media with chloramphenicol. The results showed that the method was simpler, more effective to delete target gene in genomic DNA of<I> Salmonell </I>sp. than those conventional methods.
    Keywords: temperature;Salmonella;deletion of gene;prgH genehomologous recombination;China