Le-Ying  Zhang, Jian-Guo  Li, Ling  Yang, Bin  Wang, 
    Expression of Interferon-Stimulated Gene 15 in Endometrial Explants from Early Pregnant Cows <I>in vitro</I> for 60 Days,
    Journal of Animal and Veterinary Advances,
    Volume 9,Issue 24,
    2010,
    Pages 3016-3020,
    ISSN 1680-5593,
    javaa.2010.3016.3020,
    (https://makhillpublications.co/view-article.php?doi=javaa.2010.3016.3020)
    Abstract: Interferon-tau is generally regarded as the primary signal for maternal recognition in ruminant animals. Expression of interferon-stimulated gene 15 (ISG15) is up-regulated in bovine endometrium during early pregnancy. The experimental materials were from our primary experiment. Tissue explants of endometria from day 18 of pregnant and non-pregnant cows were cultured <I>in vitro</I> for 60 days and then the expression of ISG15 in these explants was detected by western blot. The results indicated that ISG15 and conjugated proteins were still expressed at a high level in endometrial explants from pregnant cows after cultured <I>in vitro</I> for 60 days and there was no expression in endometrial explants from non-pregnant cows after cultured <I>in vitro</I> for 60 days. In conclusion, expression of ISG15 in endometrial explants from early pregnant cows maintained <I>in vitro</I> for a long time.
    Keywords: ISG1 5;primary experiment;in vitro;bovine;Endometrium;pregnant cow