Wenxiang  Zhang, Zunbao  Wang, Zongsheng  Zhao, Xiancun  Zeng, Hongbin  Wu, Peng Yu  , 
    Using Bioinpormcotics Methods to Develop EST-SSR Makers from Sheep’s ESTs,
    Journal of Animal and Veterinary Advances,
    Volume 9,Issue 21,
    2010,
    Pages 2759-2762,
    ISSN 1680-5593,
    javaa.2010.2759.2762,
    (https://makhillpublications.co/view-article.php?doi=javaa.2010.2759.2762)
    Abstract: About 29685 ESTs of sheep’s skin from NCBI were mined to develop EST-SSR markers. Totally, 1456 SSRs were identified from 1364 ESTs using SSRHunter soft tools. The frequency of EST-SSRs was 4.9%. The dinucleotide repeat motif was the most abundant SSR accounting for 32.14% followed by 28.23, 7.14, 25.21 and 7.28%, respectively for tri, hexa, penta and tetra-nucleotide repeats. Among the di-nucleotide repeats, AC/TG (58.33%) were the most abundant type. About 261 primer pairs were designed from microsatellite among which 62 pairs could show clear PCR products by electrophoresisand 5 of these primers revealed moderate to high Polymorphism Information Content (PIC) in Chinese Merino sheep. The mean PIC value was 0.6971, ranging from 0.5499-0.7440.
    Keywords: Sheep;EST;SSR;NCBI;genetic mapping;China