Darabi  Alireza, Fayazi  Jamal, Roshanfekr  Hedayatolla, Nasiri Mohammad  Taghi, 
    Investigation of Growth Hormone Gene Polymorphism Using PCR-RFLP Technique in Native Poultry in Khouzestan Province,
    Journal of Animal and Veterinary Advances,
    Volume 9,Issue 2,
    2010,
    Pages 255-257,
    ISSN 1680-5593,
    javaa.2010.255.257,
    (https://makhillpublications.co/view-article.php?doi=javaa.2010.255.257)
    Abstract: Native animal and poultry in every province are valuable genetic resourses. Khouzestan province native poultry is one of the resourses that bacuase of adaptating to environment. The growth hormone gene is a candidate genes controling the metabolic and physiologic actions. Polymorphism within this gene is a detectable parameter. In order to study and investigate growth hormone gene polymorphism, blood samples were collected from 100 individuals of native poultry and genomic DNA was extracted using salting out technique. DNA fragment with 776 bp at growth hormone gene was amplified using Polymerase Chain Reaction (PCR). The PCR product was digested with restriction enzymes MspI and subsequently electroforesed on 2.5% agarose gel with etidium bromide. The studied intron in this research was at Hardy-Weinberg disequilibrium (p<0.005). In this population, heterozygosity and effective allele were estimated 0.59 and 2.13, respectively. Obtained genotypes frequency of MspI enzyme on 776 bp fragment for AA, AB and BB genotypes were 0.39, 0.59 and 0.02, respectively. The frequency of A and B alleles were 0.685 and 0.315, respectively.
    Keywords: Growth hormone;heterozygosity;native chicken;polymorphism;gene;genotype