Makiko  Yamamoto, Yukio  Taniguchi, Koichiro  Kano, Takahisa  Yamada, 
    Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes,
    Journal of Animal and Veterinary Advances,
    Volume 9,Issue 11,
    2010,
    Pages 1639-1645,
    ISSN 1680-5593,
    javaa.2010.1639.1645,
    (https://makhillpublications.co/view-article.php?doi=javaa.2010.1639.1645)
    Abstract: Adipose depotsite-specific variability in physiological characteristics of preadipocytes could be in part, intrinsic to the cells and become evident through <I>in vitro</I> proliferation and differentiation-dependent gene expression patterns in preadipocyte in the cell culture. This study was designed to characterize proliferation and differentiation-dependent gene expression patterns intrinsic to intramuscular, perirenal and subcutaneous preadipocytes. About 3 type preadipocytes are prepared using the ceiling culture method from bovine intramuscular, perirenal and are demonstrated subcutaneous adipose depots of a single Japanese Black steer. Adipose depot site-specific proliferation and differentiation-dependent gene expression patterns intrinsic to preadipocyte by comparing the mRNA levels of CCAAT enhancer binding protein C/EBP&#945;, C/EBP&#946;, C/EBP&#948;, Peroxisome Proliferator-Activated Receptor &#947; (PPAR&#947;) and leptin before and after the induction of differentiation among culture of the 3 type preadipocytes. These results may suggest the existence of the site-specific preadipocyte-intrinsic proliferation and differentiation program.
    Keywords: Adipose depotsite;C/EBP;differentiation;gene expression;leptin;PPAR³;preadipocyte;proliferation