Kamil Uney  , Ayse Er  , Gulcan Erbil Avci  , Aziz Bulbul  , Muammer Elmas  , Enver Yazar  , 
    Effect of Tilmicosin on Serum Cytokine Levels in the Endotoxemia,
    Journal of Animal and Veterinary Advances,
    Volume 8,Issue 5,
    2009,
    Pages 1021-1024,
    ISSN 1680-5593,
    javaa.2009.1021.1024,
    (https://makhillpublications.co/view-article.php?doi=javaa.2009.1021.1024)
    Abstract: The effect of tilmicosin on serum cytokine concentrations were investigated in healthy and lipopolysaccharide-treated mice. The mice were divided into 3 groups. Lipopolysaccharide (250 &#956;g,<I> Escherichia coli</I> 0111:B4, intraperitoneally) was injected into the positive control group. The other 2 groups received tilmicosin (20 mg kg<SUP>-1</SUP>, subcutaneously) concurrently without or with lipopolysaccharide. After treatment, serum samples were collected at 0, 1.5, 3, 6, 12 and 24 h. Serum tumor necrosis factor, interleukin-1 and interleukin-10 levels were determined by enzyme-linked immunosorbent assay. Lipopolysaccharide increased all cytokine levels in the healthy mice. Tilmicosin slightly induced interleukin-1 production in the healthy mice, while it had no effect on tumor necrosis factor or interleukin-10 productions. However, tilmicosin elevated (p&lt;0.05) tumor necrosis factor, interleukin-1 and interleukin-10 levels in lipopolysaccharide-treated mice. In conclusion, these data suggest that tilmicosin stimulates both proinflammatory and antiinflammatory cytokines at the dose recommended for infection.
    Keywords: Tilmicosin;lipopolysaccharide;TNF;IL-1;IL-10