K.M. Suleima  , H. Boehnel  , S.H. Babiker  , A.Z.S.A. Zaki  , 
    Cytotoxic Activity in Fermenter Culture Supernatants of<I> Corynebacterium seudotuberculosis</I>,
    Journal of Animal and Veterinary Advances,
    Volume 5,Issue 11,
    2006,
    Pages 939-942,
    ISSN 1680-5593,
    javaa.2006.939.942,
    (https://makhillpublications.co/view-article.php?doi=javaa.2006.939.942)
    Abstract: Fermenter Culture supernatants of <I>Corynebacterium</I> <I>pseudotuberculosis</I> strain 3450 NCTC grown in a continuous culture were tested for cytotoxicity in 7 cell lines. The supernatants were toxic to EBL, MDCK, NBL, CHO-K1, 3T3, BHK and Vero cells. The cytotoxic activity was used as an assay method to determine the optimal incubation temperature and pH for cytotoxin production in fermenter. Fermenter culture supernatants of a reference strain <I>C. pseudotuberculosis</I> and three field isolates were compared for their cytotoxicity to BHK cells. Two of the field isolates were non-cytotoxic to BHK cells, one isolate had weak cytotoxicity and the reference strain was the most cytotoxic. SDS-PAGE of fermenter culture supernatants of the 4 strains revealed 35 to 39 bands with molecular weights ranging from 24 to 149 KDa.
    Keywords: Corynebacterium pseudotuberculosis;fermenter;cytotoxicity;cell lines