TY  - JOUR
T1  - Diagnosis of Phytopathogenic Fungi of the genus Fusarium in Cereals on the Territory of the
Republic of Kazakhstan using the Quantitative PCR Method
AU - Beishova, I. AU - Stakheev, A. AU - Chuzhebaeva, G. AU - Kalimov, N. AU - Kovalchuk, A. AU - Zavriev, S. 
JO  - Research Journal of Medical Sciences
VL  - 14
IS  - 6
SP  - 184
EP  - 188
PY  - 2020
DA  - 2001/08/19
SN  - 1815-9346
DO  - rjmsci.2020.184.188
UR  - https://makhillpublications.co/view-article.php?doi=rjmsci.2020.184.188
KW  - Fusarium
KW  -PCR
KW  -tef1α
KW  -cereals and primers
AB  - Diagnosis of pathogenic species of fungi of the
genus Fusarium has always been hampered by the
confusing phenotypic classification system. We used a
method based on quantitative PCR (real-time PCR) with
primers and probes developed by our laboratory which
allows to quickly and accurately identify three pathogenic
species of Fusarium fungi common in Northern
Kazakhstan: <i>F. graminearum</i>, <i>F. culmorum</i> and <i>F.
oxysporum</i>. The method is based on PCR amplification of
DNA fragments which were developed on the basis of the
translation elongation factor gene 1-&#945; (tef1&#945;). In addition
to providing accurate, reliable and rapid diagnosis of
fungi of the genus fusarium in cereals, this method
excludes the use of carcinogens (ethidium bromide).
since, quantitative PCR uses fluorescent labeled probes,
i.e., no gel electrophoresis is required.
ER  -