TY  - JOUR
T1  - Characterization of <i>Pythium aphanidermatum</i> Isolated from
Diseased Cucumber Plants in Jordan
AU - M. Alhussaen, Khalaf 
JO  - Research Journal of Applied Sciences
VL  - 14
IS  - 1
SP  - 39
EP  - 44
PY  - 2019
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2019.39.44
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2019.39.44
KW  - sequence of ITS1
KW  -root rot
KW  -cucumber
KW  -Pythium aphanidermatum
KW  -Jordan Valley
KW  -UPGMAdendrogram
AB  - The pathogen of <i>Pythium aphanidermatum</i> is a very important pathogen causing disease and yield
losses on vegetables. Correct identification of pathogens lead to correct and effective control strategies and
methods. However, the traditional methods based on morphological and physiological identification becomes
difficult and time consuming because of the similarity amongst the different groups of species and intraspecific
morphological variation frequently observed in different field isolates. Molecular techniques based on several
DNA methods have been developed to identify Pythium spp. and also to understand the relationships between
species. Morphology and physiology characteristics of <i>Pythium aphanidermatum</i> were investigated for
identification and variability. The optimum pH levels of <i>Pythium aphanidermatum</i> grow in vitro were 7 and the
optimum growing temperatures of the isolates recovery in this study was 30&deg;C. The mycelial were thick and
white with fluffy topography. Each isolate produced aseptate, hyaline mycelium, the oogonia terminal, globose
and smooth. Antheridia mostly intercalary, sometimes, broadly sac shaped, monoclinous or diclinous, thick
walled aplerotic oospores and lobed sporangia. Sequences of selected isolates obtained in this study were
matched <i>Pythium aphanidermatum</i> (KY646468) from GenBank. The UPGMA dendrogram showed that there
were variations within the population of <i>Pythium aphanidermatum</i> at similarity coefficient 0.1. These isolates
obtained from diseased cucumber plants collected from different farms in the Jordan Valley in April 2017.
ER  -