TY  - JOUR
T1  - Coliphages Lysing <I>Escherichia coli</I> Bacteria: Their Morphology and Their Response to Some Physical Factors
AU - Enan, Gamal AU - Shaaban, Khalid A. AU - Askora, Ahmed AU - Maher, Muhammad 
JO  - Research Journal of Applied Sciences
VL  - 8
IS  - 10
SP  - 516
EP  - 522
PY  - 2013
DA  - 2001/08/19
SN  - 1815-932x
DO  - rjasci.2013.516.522
UR  - https://makhillpublications.co/view-article.php?doi=rjasci.2013.516.522
KW  - E. coli
KW  -coliphages
KW  -sewage water
KW  -raw water
KW  -temperature
KW  -UV-irradiation
KW  -pH value
KW  -storage
AB  - Three coliphages designated ECP<SUB>1</SUB>, ECP<SUB>2</SUB> 
  and ECP<SUB>3</SUB> were identified in this study. Also, the effect of some 
  physical factors on the infectivity of these coliphages was studied. The three 
  coliphages were tested for their infectivity using the indicator bacterial strains 
  <I>E. coli</I> W<SUB>1</SUB> and <I>E. coli</I> W<SUB>2</SUB> where coliphage 
  ECP<SUB>1</SUB> was highly specific to infect <I>E. coli</I> W<SUB>1</SUB>, 
  on the other hand, coliphages ECP<SUB>2</SUB> and ECP<SUB>3</SUB> were highly 
  specific to infect <I>E. coli</I> W<SUB>2</SUB>. The three coliphages were very 
  sensitive to heat inactivation no survivors could be recorded after 10 min of 
  exposure time up to 60&deg;C. Also, the profile of the three coliphages inactivation 
  after different time intervals exposed to UV-irradiation at different heights 
  of 15 and 30 cm from the irradiation source was examined. Increased UV-irradiation 
  doses decreased the plaque numbers of the three coliphages tested. The effect 
  of different pH values on stability of the three coliphages was also tested. 
  Maximum stability was observed at pH 6.0. The viabilities of the three coliphages 
  were rapidly decreased towards alkalinity compared to pH 6.0. The ability of 
  the three coliphages to form plaques at preservative temperature range 3-5, 
  25-28 and 35-37&deg;C was examined. The suitable storage temperatures for the 
  three coliphages were 3-5 and 25-28&deg;C.
ER  -