TY  - JOUR
T1  - Molecular Detection and Genetic Characterization of Porcine Circovirus 3 in Yunnan, China
AU - Yin, Gefen AU - Zhang, Jintao AU - Bi, Junlong AU - Ha, Zhuo AU - Yang, Chao AU - Zeng, Caihong AU - Sun, Xianguo AU - Yang, Xiaoying AU - Yang, Runhuan AU - Lin, Yingbo AU - Xing Qu, Kai- 
JO  - Journal of Animal and Veterinary Advances
VL  - 20
IS  - 4
SP  - 91
EP  - 109
PY  - 2021
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2021.91.109
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2021.91.109
KW  - Molecular detection
KW  -phylogeny
KW  -PCV3
KW  -cap gene
KW  -Yunnan
AB  - Porcine Circovirus 3 (PCV3) infections have
been widely diagnosed and impaired the swine farm
industry worldwide. The current study carried out
molecular detection of PCV3 in 481 clinical samples
collected from 44 swine farms with reported reproductive
failures in Yunnan province of China during the period of
from 2017 to 2018. The overall PCV3 positive rate was
21.4% (103/481) with relatively low positive rate of
11.91% (28/235) in 2017 while a dramatic increase to
30.49% (75/246) in 2018 which implied the expansion of
PCV3 in Yunnan. The detection of PCV3 in pigs with no
observable clinical symptom indicated the demand of
molecular diagnostic method for more effective PCV3
control. Four entire viral genomes and 15 <i>cap</i> genes of
PCV3 strains from the positive cases were sequenced. The
phylogenetic and population expansion analyses revealed
the genetic diversity of PCV3 genome and immunogenic
<i>cap</i> gene with no obvious association with geographical
origin and pathogenic differentiation. The four Yunnan
strains fell into a mini-clade in PCV3b, indicating a novel
viral genotype. Our results contributed to better
understanding of PCV3 prevalence in Yunnan province,
China and provided supporting data for future
development of specific and effective PCV3 recombinant
vaccine.
ER  -