TY - JOUR T1 - Production of Interferon Gamma and Transforming Growth Factor Beta 1 from Bovine Mammary Gland Leukocytes AU - Slama, P. AU - Kabourkova, E. AU - Hulikova, J. JO - Journal of Animal and Veterinary Advances VL - 16 IS - 8 SP - 119 EP - 121 PY - 2017 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2017.119.121 UR - https://makhillpublications.co/view-article.php?doi=javaa.2017.119.121 KW - Inflammation KW -Escherichia coli KW -mastitis KW -dairy cattle KW -cytokines KW -incubation KW -muramy l AB - The objective of this trial was to study the Inflammatory cytokinesinterferon gamma (IFN-γ) and Transforming Growth Factor Beta1 (TGF-β1) which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 h with lipopolysaccharide (50 μg/mL) cor with muramy l dipeptide (500 μg/mL) in vitro. Lipopolyccharide was used as a toxin of Gram-negative bacteria and muramy l dipeptide as a structural unit of a peptidogly can of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher IFN-γ concentration was recorded for lipopolysacharide incubation. The production of IFN-γ started 18 h following the cell incubation with lipopolysaccharide or with muramy l dipeptide. The production of TGF-β1 was higher for lipopolysaccharide incubation than for muramy l dipeptide incubation throughout the all set time points. The TGF-β1 production started 2 h following the cell in cubation with muramy l dipeptide. ER -