TY  - JOUR
T1  - Identification of Biodiversity of Some <I>Streptomyces</I> Species and Determination of a Restriction Fragment Length Polymorphism (RFLP) Profile of <I>16S rDNA</I> Gene Region
AU - Ozdemir, K. AU - Ogun, E. AU - Ertas, M. AU - Acar, S. AU - Atalan, E. 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 16
SP  - 978
EP  - 988
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.978.988
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.978.988
KW  - Streptomyces
KW  -isolation
KW  -16S rDNA
KW  -RFLP
KW  -ratio criterion
AB  - In this study, soil samples from the roots and peripheral 
  regions of some agricultural plant species growing in the Van region (such as 
  sainfoin, bean, malus, wheat and sugar beet) were collected between May and 
  June 2004 and 139 strains of Streptomyces bacteria were isolated from these 
  samples. Moreover, researchers determined the physicochemical characteristics 
  of these soil samples. Streptomyces bacteria were color-grouped and the Diaminopimelic 
  Acid (DAP) forms were detected followed by the determination of then phenotypic 
  characteristics. When these isolates were divided into 39 color groups, 20 strains 
  were found to contain L-DAP and therefore were confirmed as members of the genus 
  <I>Streptomyces</I>. The species were identified using the TAXON Software and 
  similarity dendrograms were constructed by the Unweighted Pair-Group Method 
  with an Arithmetic average (UPGMA) cluster analysis using the Multi-Variate 
  Statistical Package (MVSP 3.1). Genomic DNA was isolated from these bacteria 
  and <I>16S rDNA</I> gene was amplified by PCR followed by restriction digestion 
  with Bsp143I, HaeIII, MnlI and SphI for Restriction Fragment Length Polymorphism 
  (RFLP) analysis. In the dendrogram obtained at the end of the RFLP analysis, 
  four groups of two or more members and six 1-member groups were established 
  according to the similarity ratio criterion.
ER  -