TY  - JOUR
T1  - Use of Random Amplified Polymorphic DNA PCR to Distinguish <I>Micromonospora</I> 
  Species Isolated from Soil
AU - Ozdemir, Kerem AU - Ertas, Metin AU - Atalan, Ekrem 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 16
SP  - 974
EP  - 977
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.974.977
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.974.977
KW  - Micromonospora
KW  -RAPD-PCR
KW  -simple matching coefficient
KW  -soil
KW  -genetic polymorphism
AB  - Recently with the advancement of molecular methods, studies 
  that determine intraspecific genetic polymorphisms have yielded substantial 
  results. The aim of this study was to use Random Amplified Polymorphic DNA PCR 
  (RAPD-PCR) analysis to determine the differences among <I>Micromonospora</I> 
  species isolated from the soil. M3 medium with antibiotics was used to isolate 
  the species from the soil and 65 <I>Micromonospora</I> species were identified. 
  Total DNA analysis was performed for the different color groups which were obtained 
  as a result analysis of the species. Sm5R, Sm6F, DAF4, M13, 27F and 1492R primers 
  were used for RAPD-PCR analysis and the band profiles of the species were obtained. 
  The dendrogram generated according to the simple matching coefficient method 
  clearly showed the differences detected among the species. A total of seven 
  groups were identified along with two main groups. Five of these groups had 
  a single member, one had 3 and one had 2 members.
ER  -