TY  - JOUR
T1  - Determination of Minimum Bactericidal Concentrations of Antimicrobials Used 
  for Canine Pyoderma Against <I>Staphylococcus pseudintermedius
AU - Harada, Kazuki AU - Iguchi, Aiko AU - Hikasa, Yoshiaki 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 15
SP  - 958
EP  - 962
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.958.962
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.958.962
KW  - Staphylococcus pseudintermedius
KW  -minimum bactericidal concentration
KW  -minimum inhibitory concentration
KW  -canine pyoderma
KW  -organism
AB  - Minimum Bactericidal Concentration (MBC) is an important <I>in 
  vitro</I> parameter of antimicrobial potency but has not yet been measured for 
  <I>Staphylococcus pseudintermedius</I>, the main causative organism of canine 
  pyoderma. This study was carried out to determine the MBC of representative 
  antimicrobials against mecA-negative and -positive <I>S. pseudintermdius</I>. 
  Fifty nine mecA-negative and 33 mecA-positive isolates were tested for Minimum 
  Inhibitory Concentration (MIC) of eight antimicrobials by Broth Microdilution 
  Method. Subsequently, MBC was determined as concentration at which &ge;99.9% 
  decrease in bacterial counts was achieved by sub-cultivating suspensions at 
  concentrations greater than MIC. As a result, cephalexin (LEX), Amoxicillin-Clavulanic 
  acid (AMC), Orbifloxacin (ORB) and Fosfomycin (FOF) had MBC:MIC ratio of &le;4 
  whereas trimethoprim-Sulfamethoxazole (SXT) had MBC:MIC ratio of &le;16. In 
  contrast, Minocycline (MIN), Erythromycin (ERY) and Clindamycin (CLI) had MBC:MIC 
  ratio of &ge;4. In mecA-negative isolates, among the tested antimicrobials, 
  FOF had the lowest MBC-resistance rate (8.5%) followed by AMC (13.6%) LEX (16.9%) 
  ORB (33.9%) SXT (33.9%) whereas MIN, ERY and CLI had high MBC-resistance rates 
  (71.2-96.6%). On the other hand, in mecA-positive isolates, all tested antimicrobials 
  had &gt;50% of MBC-resistance rates (69.7-100%). Notably, the resistant-level 
  MIC value of MIN was not detected in either mecA-negative or -positive isolates. 
  The present data indicate that FOF is a superior bactericidal drug against mecA-negative 
  isolates whereas MINO can be effective bacteriostatic drugs for mecA-positive 
  and -negative isolates of <I>S. pseudintermedius</I>.
ER  -