TY  - JOUR
T1  - Nested PCR Detection of <I>Theileria equi</I> Infection and Frequency in Horses Imported into Mexico
AU - Rivera, Theodor Duifhuis- AU - Chipres, David Sanchez- AU - Garcia, Jorge Galindo- AU - Flores, Clemente Lemus- AU - Valdovinos, Miguel Angel Ayala- AU - Estrella, Ruben Anguiano- AU - Pineda, Jacinto Banuelos- AU - Carpena, Javier German Rodriguez- 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 14
SP  - 859
EP  - 863
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.859.863
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.859.863
KW  - Theileria equi
KW  -horse
KW  -nPCR
KW  -Mexico
KW  -blood samples
AB  - The purpose of this study was to assess the frequency of <I>Theileria equi</I> in horses imported into Mexico. During different stages of quarantine, 348 blood samples were taken from clinically healthy horses imported into Mexico from 2011 to 2013. Nested PCR (nPCR) of the Merozoite Antigen-1 (<I>EMA-1</I>) gene was performed for pathogen detection. In total, 93 horses tested positive for <I>T. equi</I> resulting in a 26.72% frequency with a 95% Confidence Interval (CI) of 22.07-31.37%. This is the first molecular diagnostics study to identify <I>T. equi</I>-positive horses imported into Mexico these results highlight the importance of nPCR analysis for <I>T. equi</I> in clinically healthy imported horses.
ER  -