TY  - JOUR
T1  - Recombinant Outer Membrane Proteins (rOMPs) of <I>Brucella melitensis</I> 
  as a Potential Serological Marker for Diagnosis of Caprine Brucellosis
AU - Ahmed, I.M. AU - Khairani-Bejo, S. AU - Hassan, L. AU - Bahaman, A.R. AU - Omar, A.R. 
JO  - Journal of Animal and Veterinary Advances
VL  - 13
IS  - 4
SP  - 231
EP  - 235
PY  - 2014
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2014.231.235
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2014.231.235
KW  - Brucella melitensis
KW  -rOMPs
KW  -FPSR
KW  -ELISA
KW  -caprine brucellosis
AB  - The potential diagnostic ability of Recombinant Outer Membrane Proteins (rOMPs), a combination of equal concentrations of rOMP25, rOMP28 and rOMP31of <I>Brucella melitensis</I> was investigated using Enzyme-Linked Immunosorbent Assay (ELISA) to differentiate the False Positive Serological Reactions (FPSR) in the serological diagnosis of caprine brucellosis. The rOMPs was tested using sera from three groups of goats with known Brucella exposure status which represent, naturally <I>B. melitensis</I> infected goats (infected), Brucella free goats (non-infected) and goats vaccinated with <I>B. melitensis</I> Rev. 1 vaccine strain (vaccinated). Additionally, all the sera were tested using the common serological tests which are Rose Bengal Plate Test (RBPT), BRUCELISA-400SG and Complement Fixation Test (CFT). When testing infected and non infected groups, the rOMPs I-ELISA recorded 94.44% (34/36) sensitivity and 100% (36/36) specificity and this almost agreed with the results obtained from testing the same serum samples using RBPT, BRUCELISA-400SG and CFT. However, when goats vaccinated with <I>B. melitensis</I> Rev. 1 vaccine strain were tested by the common serological tests, RBPT, BRUCELISA-400SG and CFT they wrongly recorded positive results for all the tested serum samples (26/26). While the developed rOMPs I-ELISA was able to differentiate the vaccinated from infected animals with 94.44 sensitivity and 84.62% specificity. The potential diagnostic ability of rOMPs would be of great importance as serologic marker to minimize the FPSR in eradication programs of caprine brucellosis.
ER  -