TY  - JOUR
T1  - Construction and Identification of Prokaryotic and Eukaryotic Expression Vectors of Porcine Stratifin
AU - Xu, Zhiwen AU - He, YaLin AU - Liu, Mengliang AU - Wang, XiaoDi AU - Xu, ShanShan 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 10
SP  - 965
EP  - 971
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.965.971
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.965.971
KW  - Porcine stratifin
KW  -prokaryotic expression
KW  -14-3-3 protein
KW  -brain
KW  -organisms
AB  - Prokaryotic expression vector of porcine stratifin was constructed 
  to express stratifin in <I>Escherichia coli</I> and Eukaryotic expression vector 
  was also constructed. Stratifin cDNA was amplified using the PCR and cloned 
  into PET32a (+) vector to form the recombinant plasmid SFN-PET32a (+) the recombinant 
  plasmid SFN-PET32a (+) was then transformed into prokaryotic expression host 
  <I>E. coli </I>Rosetta using lactose induction; the target protein was purified 
  by using His affinity chromatographic separation. The recombinant protein stratifin 
  was identified by SDS-PAGE. Stratifin cDNA was inserted into PCDNA3.1 (+) to 
  form the recombinant plasmid SFN-PCDNA3.1 (+) and then it was identified by 
  restriction enzyme digestion and DNA sequencing. The recombinant plasmid stratifin 
  was successfully constructed, the recombinant stratifin protein was induced 
  and eukaryotic expression vector was constructed. This study successfully realizes 
  the construction of the prokaryotic and eukaryotic expression vectors and it 
  could be used as a foundation for the future research on the function of this 
  protein.
ER  -