TY  - JOUR
T1  - Combinational Role of Oxidant Response Genes <I>rpoS</I>, <I>ssrAB</I> and 
  <I>hmp</I> in the Virulence of Highly Pathogenic <I>Salmonella typhimurium</I> Isolated in Korea
AU - Cho, Youngjae AU - Lee, Mi Rae AU - Park, Yoon Mee AU - Park, Hee Jeong AU - Bang, Iel Soo AU - Hahn, Tae-Wook 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 8
SP  - 868
EP  - 875
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.868.875
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.868.875
KW  - Live attenuated vaccine
KW  -oxidant response genes
KW  -Salmonella typhimurium
KW  -virulence
KW  -rpoS mutant
AB  - This study was designed to elucidate the role of oxidant response 
  genes in the virulence of attenuated <I>Salmonella typhimurium</I> (ST). After 
  ST mutants with single or multiple deletions were constructed: the susceptibility 
  of ST to hydrogen peroxide and S-nitrosoglutathione, expression of Salmonella 
  pathogenicity island-2 genes and the virulence of attenuated ST were assessed 
  in this study. Unexpectedly, no difference was observed between the growth of 
  rpoE mutant ST98 and Wild Type (WT) however, the rpoS mutant was hypersusceptible 
  to hydrogen peroxide. The expression and Nitrogen Oxide (NO)-detoxifying activity 
  of the flavohemoglobin hmp were associated with the mechanism of resistance 
  of ST98 to NO. A triple mutant of ST98 in which <I>rpoS</I>,<I> ssrAB</I> and 
  <I>hmp</I> genes were deleted showed a permanent phenotype with no modification 
  of other characteristics. In a mouse infection test, the survival of ST98 triple 
  mutant was lower than that of WT. The virulence of ST was shown to be associated 
  with the oxidant response genes. This study provides useful information for 
  understanding the combinational role of oxidant response genes in the virulence 
  of Salmonella.
ER  -