TY  - JOUR
T1  - Molecular Identification and Expression Analysis of the <I>Interferon Beta</I> Gene in Sheep (<I>Ovis aries</I>)
AU - Zhang, Hua-Tang AU - Gao, Hua-Feng AU - Li, Ming-Li AU - Xu, Wen-Wen AU - Yang, Shi-Biao AU - Xin, Ai-Guo AU - Li, Hua-Chun 
JO  - Journal of Animal and Veterinary Advances
VL  - 12
IS  - 3
SP  - 412
EP  - 418
PY  - 2013
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2013.412.418
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2013.412.418
KW  - Sheep
KW  -interferon beta
KW  -tissue distribution
KW  -chelate
KW  -amino acid
AB  - Interferon beta (IFN-&#946;) belongs to the group of inflammatory cytokines that are expressed in eukaryotic cells as an early response to viral infection. Sheep as an important livestock in the world, however, little is known about sheep Interferon beta (sIFN-&#946;) and its role in immune responses. In this study, researchers amplified the cDNA of sIFN-&#946; from sheep spleen by RT-PCR using primers according to the cattle IFN-&#946;. The ORF of sIFN-&#946; covers 561 bp encoding 186 amino acids. Sequence prediction shows that the amino acid of sIFN-&#946; possess 22 aa signal peptide domain and two putative N-glycosylationlation sites. qPCR analysis indicated that sIFN-&#946; mRNA was predominantly expressed in lymphoid node. The 36 kDa recombinant sIFN-&#946; protein was efficiently expressed and purified using metal chelate affinity chromatography (Ni-NTA) then confirmed by SDS-PAGE and Western blotting. This study provides a deeper understanding of sIFN-&#946; molecule structure and also contributes to further studies.
ER  -