TY - JOUR T1 - Phylogenetic Analysis of the Streptococcus agalactiae Sip Gene in China AU - Xingxu, Zhao AU - Jie, Fan AU - Yong, Zhang JO - Journal of Animal and Veterinary Advances VL - 12 IS - 17 SP - 1395 EP - 1398 PY - 2013 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2013.1395.1398 UR - https://makhillpublications.co/view-article.php?doi=javaa.2013.1395.1398 KW - Streptococcus agalactiae KW -Sip KW -phylogenetic KW -amino acid KW -China AB - A Streptococcus agalactiae GS was isolated from milk from mastitis cattle in Gansu region of China. Sequence and phylogenetic analysis of Sip gene segments revealed that the Streptococcus agalactiae GS was most similar to a recent group B streptococcus isolated in dairy cattle from China. The Sip gene were selected and amplified, it contained a 1305 bp Open Reading Frame (ORF) which encoded 434 amino acids. The molecular mass of the deduced amino acid sequences was 56 kD. Blast analysis showed that it shared high identities (91.0-99.0%) with Sip sequences of other GBS registered in GenBank while lower identities (<50%) was found compared with other species of Streptococcus. The deduced amino acid homology was 90.2-98.8%. ER -