TY  - JOUR
T1  - Polymorphism Analysis on the Second Intron of the <I>GH</I> Gene in Chinese Donkeys
AU - Zhu, Wenjin AU - Su, Yongmei AU - Ma, Yanhua AU - Ni, Jing AU - Guan, Xuemin AU - Wu, Jianhua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 7
SP  - 916
EP  - 918
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.916.918
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.916.918
KW  - Donkey
KW  -GH gene
KW  -PCR-SSCP
KW  -genetics diversity
KW  -mutation
KW  -polymorphisms
AB  - To reveal the genetical diversity of different donkey breeds at molecular level and provid some bases for the breed resource evaluation and utilization of donkeys in China, polymorphism of the second intron of the <I>GH </I>gene was analysised by PCR-SSCP in the seven donkey breeds (LX, GZ, XJ, GL, HB, DZ, JN). The results showed three haplotypes with the percentage of 1.7% in 174 samples were obtained, the haplotype diversity was high as 0.678 and 0.542 for the Linxian and Huaibei donkey and low as 0.077 for Xingiiang donkey with that of 0.409 and 0.462 for Guangling donkey and Guanzhong donkey, respectively with that of 0.355 and 0.304 for Dezhou donkeys and Jinnan donkeys, respectively. The amplified fragments of A and B haplotypes were cloned and sequenced. The result showed the fragments of B haplotypes had one substitution mutation at 735 site (G&rarr;C), the fragments of A haplotypes had one substitution mutation at 869 site (G&rarr;T). The results confirmed that there were polymorphisms in the second intron (214 bp) of donkey <I>GH </I>gene for the first time.
ER  -