TY  - JOUR
T1  - New SNPs of the Duck <I>LPL</I> Gene are Associated with Body Weight, Fatness and Carcass Traits
AU - Yang, Yongping AU - Gong, Ping AU - Li, Shijun AU - Peng, Xiuli AU - Feng, Yanping AU - Gong, Yanzhang 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 5
SP  - 578
EP  - 582
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.578.582
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.578.582
KW  - Duck
KW  -PCR-SSCP
KW  -LPL
KW  -SNP
KW  -body weight
KW  -fatness
KW  -carcass
KW  -PCR-RFLP
AB  - Lipoprotein Lipase (LPL) is a multifunctional protein that plays a major role in the hydrolysis of triglycerides present in chylomicrons and very low-density lipoproteins. This study was designed to investigate the effects of Single Nucleotide Polymorphisms (SNPs) of the duck <I>LPL</I> gene on fatness and carcass and growth traits. A White Kaiya x White Liancheng F<SUB>2</SUB> population with a total of 1069 individuals was used in the present study, 440 healthy ducks randomly selected from this population were slaughtered at 80 days of age. PCR-Single Strand Conformation Polymorphism (PCR-SSCP) and sequencing methods were used to detect SNPs in the duck <I>LPL</I> gene. Two new SNPs (C645T and G726A) were discovered in exons 5 and genotyped using the PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) Method. Genotyping results showed that the genotype distribution differed between males and females, thus the association analyses were performed separately for males and females. Association analyses indicated that the SNPs were significantly associated with body weight, fatness and carcass traits (p&lt;0.05 or p&lt;0.01). We concluded that LPL is a major gene or is linked with a major gene that influences body weight, fatness and carcass traits and that C645T and G726A could be used as candidate molecular genetic markers for breeding selection.
ER  -