TY  - JOUR
T1  - Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing the Rabies Virus Glycoprotein and EGFP
AU - Hu, Rongliang AU - Han, Naijun AU - Li, Yewei AU - Sun, Chenglong AU - Meng, Qingguo AU - Song, Feifei AU - Yang, Yang AU - Zhang, Shoufeng 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 4
SP  - 566
EP  - 571
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.566.571
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.566.571
KW  - Rabies Virus (RV)
KW  -Pseudorabies Virus (PRV)
KW  -UL21 gene
KW  -SRV9 glycoprotein
KW  -oral vaccine
KW  -homologous recombination
AB  - The rabies virus glycoprotein is an efficient vaccine antigen particularly for the oral vaccine of wildlife. Pseudorabies virus contains many non-essential genes and was usually used to construct recombinant vaccines as a viral vector to deliver foreign genes into the target animals. In this study, a recombinant pseudorabies virus rPRV/SRV9G/EGFP, expressing EGFP and rabies virus (SRV9 strain) glycoprotein was constructed by homologous recombination in the <I>UL21</I> gene. The immunogenicity was evaluated as a vaccine in mice. In this trial, thirty mice were administered with the recombinant vaccine orally. This recombinant virus was demonstrated to be safe for mice by oral administration and could induce neutralizing antibody level above 0.5 IU mL<SUP>-1</SUP> at 3 weeks after the vaccination. The results suggest that rPRV/SRV9G/EGFP was an effective oral vaccine candidate against rabies and <I>UL21</I> gene could be a target insertion area for foreign genes.
ER  -