TY  - JOUR
T1  - Ontogenic Expression Pattern and Genetic Polymorphism of the Peroxisome Proliferator-Activated Receptor (<I>PPAR-&#947;</I>) Gene in Goose Populations
AU - He, Da-Qian AU - Wu, Hua-Li AU - Wang, Hui-Ying AU - Liu, Yi AU - Gong, Shao-Ming AU - Kong, Fan-Li AU - Zhu, Qing 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 24
SP  - 4625
EP  - 4631
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4625.4631
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4625.4631
KW  - Goose
KW  -PPAR-y gene
KW  -SNP
KW  -carcass traits
KW  -expression
AB  - Peroxisome Proliferator-Activated Receptor &#947; (PPAR-&#947;) 
  was a key regulator of proliferation and preadipocyte differentiation in mammals. 
  The objective of this study was to investigate the effect of the <I>PPAR-&#947; 
  </I>gene on slaughter traits of 170 individuals from Zhejiang White geese (ZW) 
  and the ontogenetic expression pattern. PCR-SSCP technique was developed to 
  analyze coding region of the <I>PPAR-&#947;</I> gene polymorphism. The results 
  showed that two genotypes (AA and AG) which were the products of two alleles 
  (A and G) were observed. Association analysis indicated that this SNP was significantly 
  associated with percentage of goose leg muscle (p&lt;0.05) and the allele A 
  was the predominant allele. Quantitative real-time PCR (qRT-PCR) assay measured 
  the PPAR-&#947; mRNA expression in ZW and Landes goose five tissues of different 
  ages (P1, P14, P21, P28, P35, P42, P49, P56 and P63). The results showed that 
  the PPAR-&#947; mRNA had the highest expression level in goose abdominal fat 
  and subcutaneous fat followed by lung and intestine. The PPAR-&#947; mRNA levels 
  exhibited a rise-decline change in fat tissues except for ZW subcutaneous fat. 
  The values in fat tissues were higher than those of other tissues at the same 
  goose breed (p&lt;0.01). Besides, average values in ZW fat and liver tissues 
  were higher than those of Landes goose totally. These results suggested that 
  the PPAR-&#947; expressions were positively associated with goose fat development 
  and had a breed-related tendency. The <I>PPAR-&#947;</I> gene polymorphism could 
  be used in Marker Assistant Selection (MAS) as a genetic marker for goose slaughter 
  traits.
ER  -