TY - JOUR
T1 - The Effects of Interferon-Gamma (IFN-γ) on the Expression of IL-10
in Hypothalamic Pituitary Ovarian Axis and Peripheral Blood
AU - Si, Lifang AU - Xue, Yun AU - Cheng, Xiangchao AU - Gao, Chunsheng AU - Zhao, Zhanqin AU - Chen, Shulin AU - Zhao, Jin
JO - Journal of Animal and Veterinary Advances
VL - 11
IS - 23
SP - 4310
EP - 4314
PY - 2012
DA - 2001/08/19
SN - 1680-5593
DO - javaa.2012.4310.4314
UR - https://makhillpublications.co/view-article.php?doi=javaa.2012.4310.4314
KW - IL-10
KW -IFN-y
KW -immunohistochemical
KW -hypothalamic pituitary ovarian axis
KW -pregnant rats
AB - The aim of this research was to confirm the effect of IFN-γ
on the expression level of IL-10 in hypothalamic pituitary ovarian axis and
peripheral blood of rats during early pregnancy. The rats (gestational day 9)
were randomly divided into 3 groups: test group 1 (vaginal muscular injection
with 2.5x104 U IFN-γ per rat), test group 2 (vaginal muscular
injection with 7.5x104 U IFN-γ per rat) and control group (vaginal
muscular injection with normal saline). About 48 h after injection, the hypothalamus,
pituitary, ovary, uterus and peripheral blood were collected to research the
effect of exogenous IFN-γ on the expression level of IL-10 by morphological
observation, immunohistochemical SP and ELISA. The results showed that injection
of IFN-γ (in the dose of both 2.5x104 U and 7.5x104
U per rat) decreased the expression of IL-10 in hypothalamic periventricular
nucleus, hypothalamic supraoptic nucleus, nucleus pre-opticus magno cellularis,
medial preoptic nucleus, lateral preoptic nucleus, suprachiasmatic nucleus,
nucleus arcuatus hypothalamic, adenohypophysis, corpus luteum, stromal cells
in uterine decidua, utricular glands and peripheral blood. These findings suggest
that IFN-γ could down regulate the expression of IL-10 in hypothalamic-pituitary-ovarian
axis and peripheral blood at a does independent manner.
ER -