TY - JOUR T1 - Characterization of PB-CS01, A Novel Photobacterium Strain Isolated from Commercial Pork AU - Chen, Xiaojun AU - Kuang, Guangwei AU - Xiao, Andong AU - Chen, Qiyou AU - Zhang, Dasheng AU - Wang, Hui AU - Sun, Zhiliang JO - Journal of Animal and Veterinary Advances VL - 11 IS - 23 SP - 4292 EP - 4296 PY - 2012 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2012.4292.4296 UR - https://makhillpublications.co/view-article.php?doi=javaa.2012.4292.4296 KW - Photobacterium KW -PB-CS01 KW -phenotype KW -phylogeny KW -species AB - A Gram-negative, rod-shaped bacterial strain named PB-CS01 which is bioluminescence-positive was isolated from contaminated commercial pork that probably had been exposed to seafood. Optimal growth of strain PB-CS01 requires the presence of 3.0% (w/v) NaCl and a temperature of 20°C. Phylogenetic analysis based on 16S rRNA gene sequences of strain PB-CS01 and other Photobacterium species showed that the novel isolate belongs to the genus Photobacterium. Sequence similarity analysis between PB-CS01 and other species indicates that the closest relatives of strain PB-CS01 are Photobacterium phosphoreum ATCC 11040 (99.9%), Photobacterium kishitanii pjapo.1.1 (99.8%) and Photobacterium iliopiscarium ATCC 51760 (99.5%). The most abundant fatty acids were summed feature 3 (50.77%; C16:1ω7c and/or C16:1ω6c) and C16:0 (15%). The fatty acid profile is similar to that of the genus Photobacterium but this report is the first to describe C16:1ω6c as one of the compositions of summed feature 3 of the genus Photobacterium. The G+C content of the genomic DNA of strain PB-CS01 was 44.8 mol%. Overall, strain PB-CS01 is a novel Photobacterium species. ER -