TY  - JOUR
T1  - Detection of Pathogenic Marek’s 
  Disease Virus by Dot Blot Hybridization and PCR in Commercial Chickens in China
AU - Ding, Jiabo AU - Chen, Ruiai AU - Guo, Kai AU - Wang, Nan AU - Gu, Jinhua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4250
EP  - 4257
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4250.4257
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4250.4257
KW  - Detection
KW  -Marek`s disease virus
KW  -dot blot hybridization
KW  -PCR
KW  -China
AB  - Marek’s Disease 
  Virus (MDV) is an oncogenic herpesvirus that causes various clinical syndromes 
  in chickens which lead to huge economic losses for poultry industry. Compared 
  with virulent MDV (vMDV) strains it was found that the vaccine strain CVI988 
  had a 5 bp deletion in the bi-directional promoter region in the genome of MDV. 
  Based on the deletion, researchers developed a Polymerase Chain Reaction (PCR) 
  for the rapid identification of vMDV strains from vaccine CVI988 strain. In 
  the present study, the sensitivity and specificity of different methods including 
  PCR, virus isolation and dot blot hybridization for detecting MDV were compared. 
  The results showed that PCR and dot blot hybridization assays were comparable 
  in sensitivity to virus isolation and there were good correlations in positive 
  results among the three techniques. In addition, the PCR assay similar with 
  virus isolation was specific enough to identify CVI988 vaccine strain from vMDV. 
  In company with PCR, the dot blot hybridization with the probe of <I>pp38</I> 
  gene labeled with digoxigenin were used to monitor the vMDV infection in commercial 
  chicken flocks in China. The data demonstrated that MDV were widespread in commercial 
  chickens in China and the combination of dot blot hybridization and PCR assay 
  was useful for screening for wild vMDV strains infection in chickens.
ER  -