TY  - JOUR
T1  - The Cloning <I>F</I> Gene of Pigeon Paramyxovirus TypeI (PPMV-1) PL Strain and the Study on DNA Vaccine
AU - Yu, SiJiu AU - He, FenYi AU - He, JiongJie AU - Guo, HuiLin AU - Yang, Ming AU - Chen, ZhiHua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4210
EP  - 4216
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4210.4216
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4210.4216
KW  - PPMV-1
KW  -F gene
KW  -cloning
KW  -NDV
KW  -pigeon
AB  - One pair of primers were designed based on <I>F</I> gene of 
  Pigeon Paramyxovirus type I (PPMV-1) sequences reported in GenBank. <I>F</I> 
  gene fragment about 1.66 kb was amplified by Reverse Transcription Polymerase 
  Chain Reaction (RT-PCR) with the genome of Newcastle Disease Virus (NDV) PL 
  strain as the template. Sequence analysis showed that <I>F</I> gene of PL strain 
  shared 76.3-98.6% homology with the nucleotide sequence of <I>F</I> gene of 
  domestic and foreign 17 strains PPMV-1 or NDV. The <I>F</I> gene of PL strain 
  was inserted into eukaryotic expression vector PCDNA3.1V5HIS so eukaryotic expressing 
  plasmid PCDNA3.1-PPMV-1-F was constructed. Researchers immunized some 1 month 
  old young pigeons which were not immunized against NDV with the constructed 
  eukaryotic expression recombinant plasmid. The dose was 100 &#956;g/feather. 
  After 2 weeks, researchers boosted immunization one time. Respectively, collected 
  blood from vein under the wings and separated serum on days 0, 7, 14, 21 and 
  28 after booster immunization. The blood serum antibody titers of different 
  groups were assayed by indirect ELISA. The results showed that the antibodies 
  producing after immunizing young pigeons with eukaryotic expression plasmid 
  specifically reacted with F protein of PPMV-1. The antibody began to produce 
  on day 7 after immunization were maximum on day 14 and then the level began 
  to decline. This indicated that F protein had good immunogenicity. All young 
  pigeons were challenged with 100 times 50% Egg Infections Dose (EID50) of virus 
  homologous to <I>F</I> gene on day 28 after immunization with eukaryotic expression 
  recombinant plasmid. The results showed that the protective rate of immunized 
  with recombinant plasmid group and immunized with pigeon NDV propolis inactivated 
  vaccine group were respectively, 3.3 and 100% significantly higher than physiological 
  saline group and PCDNA3.1V5HIS mock-vehicle group. The protective rate of pigeon 
  NDV propolis inactivated vaccine group was also higher than the recombinant 
  plasmid group. This indicated that the eukaryotic expressing plasmid of <I>F 
  </I>gene constructed by us as candidated genetic vaccines could induce young 
  pigeons to produce protective immune response but it also needed other methods 
  to raise the efficiency of immunity.
ER  -