TY  - JOUR
T1  - Effect of Mitochondrial Dysfunction in Nuclear Donor and Ooplasmic Recipient on Early Development of Goat-Sheep Interspecies Somatic Nuclear Transfer Embryos
AU - Zhang, Yanru AU - Cao, Junwei AU - Ma, Libing AU - Song, Hua AU - Zhou, Huanmin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 22
SP  - 4079
EP  - 4085
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4079.4085
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4079.4085
KW  - Mitochondria
KW  -interspecies somatic nuclear transfer
KW  -goat
KW  -sheep
KW  -donor cells
KW  -China
AB  - Mitochondria is a kind of important organelle for producing 
  and supplying energy in eukaryotes. The interspecies somatic nuclear transfer 
  could result in coexisting of nuclear donor and ooplasmic recipient mitochondria 
  in reconstructed embryos. In the present study, after nuclear donor and/or ooplasmic 
  recipient mitochondria damaged by photoirradiating rhodamine-123, examination 
  of the goat-sheep interspecies somatic nuclear transfer embryos early development 
  ability <I>in vitro</I> was performed. The result shows that sheep ooplasmic 
  recipient mitochondria damaged could result in reconstructed embryos development 
  ability declined in 2-cell and morulae/blastocyst stage but does not affect 
  2-cells stage to 8-cells stage development; goat nuclear donor cells mitochondria 
  damaged can not affect reconstructed embryos development ability before morulae/blastocyst 
  stage; after both nuclear donor and ooplasmic recipient mitochondria are damaged, 
  the reconstructed embryos development pattern is same as that of only ooplasmic 
  recipient mitochondrial damaged. The result indicated that before interspecies 
  somatic nuclear transfer reconstructed embryo development to morulae/blastocyst 
  stage, ooplasmic recipient mitochondrial is dominant over nuclear donor mitochondrial 
  in affecting reconstructed embryos development ability.
ER  -