TY  - JOUR
T1  - Biochemical Characterization of the Outer Membrane Enzyme OMPLA from <I>Riemerella anatipestifer</I>
AU - Sun, Mingfei AU - Liao, Shenquan AU - Yuan, Jianfeng AU - Qin, Zonghua AU - Wu, Caiyan AU - Lv, Minna AU - Qi, Nanshan AU - Cai, Jianping 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 21
SP  - 4073
EP  - 4078
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4073.4078
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4073.4078
KW  - Riemerella anatipestifer
KW  -outer membrane phospholipase A
KW  -methyl arachidonyl fluorophosphonate
KW  -protein
KW  -database
AB  - A gene (<I>pldA</I>) encoding a 33.0 kDa protein with significant homology to the <I>Escherichia coli</I> outer membrane phospholipase A was identified from <I>Riemerella anatipestifer</I> genome database. The results of this study revealed that this gene encoded an Outer Membrane Phospholipase A (OMPLA) in <I>Riemerella anatipestifer</I>. The recombinant OMPLA protein was expressed and its general biochemical features were also, determined. Methyl Arachidonyl Fluorophosphonate (MAFP) could specifically inhibit the OMPLA activity. These observations suggest that OMPLA could be exploited as a drug target in the bacteria and MAFP may serve as a lead compound for drug discovery against <I>R. anatipestifer</I>.
ER  -